transgenic cells

transgenic cells

Beside our customized antibody generation systems, we provide the genetic engineering of mammalian cells. Initially we optimize your sequence of interest with the help of bioinformatics tools e.g. Kozak consensus sequence. In use of a transposon related expression vector we stably insert your genes of interest and obtain transgenic cell lines expressing the requested protein. After a strong puromycin-based selection we provide cells expressing the protein to 100%. We validate the cells via SDS-Page, Western Blot, Confocal-Microscopy or other application discussed before in line with our clients. We ship the cells with a detailed cultivation protocol and validation report.